Part:BBa_K2997006:Design
K880005 - yebF - TB linker - sCBM-B
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 169
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In the human gut microbiome, clostridium related species have been reported to have the highest production ability of p-cresol from tyrosine[1]. We decide to take c.diff as a model of p-cresol producer because it is a popular research target due to its notorious infectious ability.
Luckily, igem ncku 2019 was kindly supported by one of our instructor PI, who is currently devoting himself in the field of developing novel therapeutic approach for c.diff infection. And he kindly provide us a plasmid cloned with a bacteriocin gene(CBM-B) from CBM, which has been proven to have the bactericidal ability to certain strain of clostridium, including c.diff[2].
In the original designs for inhibiting clostridium strains and thus lowering p-cresol production in gut, bacteriocin is fused with a secretion tag, yebF. YebF is a secretory protein with unknown function in e.coli[3]. We first amplify the yebF fragments from the genome of e.coli MG1655 and bacteriocin
Source
In the human gut microbiome, clostridium related species have been reported to have the highest production ability of p-cresol from tyrosine[1]. We decide to take c.diff as a model of p-cresol producer because it is a popular research target due to its notorious infectious ability.
Luckily, igem ncku 2019 was kindly supported by one of our instructor PI, who is currently devoting himself in the field of developing novel therapeutic approach for c.diff infection. And he kindly provide us a plasmid cloned with a bacteriocin gene(CBM-B) from CBM, which has been proven to have the bactericidal ability to certain strain of clostridium, including c.diff[2].
In the original designs for inhibiting clostridium strains and thus lowering p-cresol production in gut, bacteriocin is fused with a secretion tag, yebF. YebF is a secretory protein with unknown function in e.coli[3]. We first amplify the yebF fragments from the genome of e.coli MG1655 and bacteriocin